Institute for Translational Brain Research - ShuLab
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  • Laboratory of Neural Excitability and Neuromodulation
  • Institute for Translational Brain Research,
  • State Key Laboratory of Medical Neurobiology,
  • MOE Frontiers Center for Brain Science,
  • Department of Neurology, Huashan Hospital
  • Fudan University
  • Lab Head: Yousheng Shu
    Email: yousheng@fudan.edu.cn
  • The Fudan University Fenglin Campus, 131 Dong'an Road, Xuhui District, Shanghai

    • 1. Collect discarded brain tissue from patients during neurosurgery.

    • 2. Block the tissue.


    • 3. Cut thin slices at 0 degree.

    • 4. Incubate slices at 35 degree.


    • 5. Visualize slices in a submerge chamber.

    • 6. Record at body temperature.

7. Cortical slices that maintained in modified ACSF can generate spontaneous recurrent network activities (Sanchez-Vives and McCormick, 2000 Nat. Neurosci.; Shu et al., 2003 Nature). 

Copyright 2020, all rights reserved. Laboratory of Neural Excitability and Neuromodulation，Institute for Translational Brain Research, Fudan University.
Designed by Jun Yang: yangjun85824@163.com. Updated by Suixin Deng: sxdeng@mail.bnu.edu.cn.