- A list of equipment for a slice recording rig:
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1. Up-right microscope;
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2. Infrared CCD camera;
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3. Monitor;
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4. Manipulator and stands;
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5. X-Y translator for microscope;
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6. Isolation table;
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7. Recording chamber with perfusion system;
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8. Temperature controller;
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9. Patch clamp amplifier;
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10. Data acquisition system;
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11. Oscilloscope;
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12. Stimulator;
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13. Computer;
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14. Pipette puller;
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15. Slicer (or vibratom).
Olympus BX51WI Infrared Light Differential Interference Contrast (IR-DIC) Microscope.
Olympus FV1200-MPE Two-photon Microscope.
Custom-built Random Scanning Two-photon Microscope.
This system was built by Prof. Shaoqun Zeng and his
group at Britton Chance Center for Biomedical Photonics, Wuhan National
Laboratory for Optoelectronics–Huazhong University of Science and
Technology.